Site-specific Endonucleases inStreptomycesStrains
نویسندگان
چکیده
منابع مشابه
Reaction kinetics of some important site-specific endonucleases
Reaction kinetics of the site-specific endonucleases BamHI, BgIII, C1aI, EcoRI, HpaII, PstI, SaII, SmaI, and XorII were investigated employing some frequently used substrates. Six of these enzymes could be analyzed under steady-state conditions. Kinetic data were obtained from progress curves applying an integrated Michaelis-Menten equation. KM ranged from 4 x 10(-9) M to 4 x 10(-11) M. Activit...
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Four site-specific endodeoxyribonucleases have been partially purified from extracts of bacteriophage T5-infected Escherichia coli by gel filtration and affinity chromatography on single- and double-stranded DNA. The enzymes were detected and characterized by agarose gel electrophoresis of alkali-denatured digestion products. None of the four is found in uninfected cells. In the presence of a d...
متن کاملSite-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.
We present in Table I an updated list of the sensitivities of over 240 restriction endonucleases to the site-specific DNA modifications '14C, n-6C, h1"5C, and m6A, four modifications that are common in DNA prokaryotes, eukaryotes, and their viruses (Mc2,Mc5,Mc8,Mcl 1 ,Ne3,Ne4). Table II is a list of over 130 characterized DNA methyltransferases. A detailed list of cloned restrictionmodification...
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In somatic mammalian cells, homologous recombination is a rare event. To study the effects of chromosomal breaks on frequency of homologous recombination, site-specific endonucleases were introduced into human cells by electroporation. Cell lines with a partial duplication within the HPRT (hypoxanthine phosphoribosyltransferase) gene were created through gene targeting. Homologous intrachromoso...
متن کاملEffect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases
Restriction endonucleases have site-specific interactions with DNA that can often be inhibited by site-specific DNA methylation and other site-specific DNA modifications. However, such inhibition cannot generally be predicted. The empirically acquired data on these effects are tabulated for over 320 restriction endonucleases. In addition, a table of known site-specific DNA modification methyltr...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1980
ISSN: 0002-1369
DOI: 10.1080/00021369.1980.10864188